Sentence examples for buffer in the absence from inspiring English sources

The blank reaction consisted of 50 mM phosphate buffer in the absence of leaf extracts.

Activation can occur in a simple Tris-HCl buffer, in the absence of added divalent cations and in the presence of EDTA.

To determine whether EPO is cardioprotective, isolated rat hearts were perfused for 10 min in the Langendorff-mode with Krebs-Henseleit buffer in the absence or presence of brief recombinant EPO treatment while left-ventricular-developed pressure (LVDP) was measured continuously to assess contractile function.

The experiments performed under non-reducing conditions were performed in the same buffer, in the absence of DTT.

Control experiments were carried out in the same buffer in the absence of PO43− or in the presence of 20 mM SO43−.

The agarose beads were then washed four times with 1 ml each of HEMG buffer in the absence of protease inhibitor and bovine serum albumin.

The background of the corresponding buffers in the absence of protein and D-ribose was subtracted for all the samples.

Reducing the reaction temperature from 60° to 20 °C in Tris-buffer in the absence of organic co-solvent had a similar effect (eeP 25%% versus 52%%, respectively).

These were studied in KH buffer in both the absence and presence of isoproterenol at a concentration (100 m m) at which it would be possible to assess the extent to which the RyR2 p/s or RyR2 s/s mutation might either increase or decrease the incidence of ectopic or subsidiary Ca2+ events.

Controls were placed into a similar buffer solution in the absence of Sulfo-NHS and EDC.

Thermal stability was measured by incubating the enzyme (100 µg/ml) at given temperatures in buffer B, in the absence and in the presence of ligands.