Cell pellets were resuspended in 10 mM imidazole buffer (3 4 buffer excess in relation to wet biomass).
The following remarkable conclusion follows: the buffer capacities towards different components are reciprocally proportional, while the buffer capacities in relation to the ions of the solid phase are interconnected through its stoichiometric coefficients.
The same soil can possess different buffer action in relation to different metals.
The buffer properties in relation to the solid phase components are amplified with an increase of solubility due to protolytic or complex formation equilibria in saturated solutions.
It is established that buffer properties in relation to components of the solid phase amplify with the increase of the solubility.
On the basis of the written equations, it is possible to deduce the formulas for calculating the buffer capacity in relation to any component of the mixture (Povar and Spinu 2013a): frac{beta_A^S}{n^2}=frac{beta_M^S}{m^2} (15).
In a second experiment, three random native rabbit samples were serially diluted with assay buffer and measured in relation to serial dilutions of the reference materials (recombinant rabbit IGF-I and recombinant human IGF-I).
A few studies provide evidence that the overexpression of CaBP-D28K may be neuroprotective (Yuan et al. 2013) and that this cytoplasmatic Ca-buffer is regulated in relation to neuronal activity (Baimbridge et al. 1985).
For example, if in a station a buffer is expected to carry the same load (which cannot be calculated in advance) with another buffer of a higher priority, then we cannot estimate based on (5) at what degree the second buffer will be favored in relation to the first one.
The buffer capacity of soils can be discovered by the fact that the increase of amounts of toxic metals is not accompanied by an increase of their content in plants; the different buffer action of the soils in relation to one element is manifested in unequal toxic concentrations for plants.
