Sentence examples for buffer immunoprecipitates were eluted from inspiring English sources

After six rinses with ice cold ChIP buffer, immunoprecipitates were eluted from the beads with two successive additions of 125 µl freshly made elution buffer (0.2% SDS, 0.1 M NaHCO3, 5 mM DTT) incubated at 65°C for 10 min. Eluates were combined, and adjusted with Tris and EDTA to a final concentration of 10 mM Tris pH8 and 2 mM EDTA.

After washing in IP buffer, immunoprecipitates were eluted in loading buffer (60 mM Tris.Cl pH 6.8, 1% SDS, 100 mM DTT, 5% glycerol) prior to western blotting.

Beads were then washed 5 times with IP lysis buffer and immunoprecipitates were eluted in Laemmli buffer at 99 °C for 10 min. For immunoprecipitation experiments of mitochondrial and cytosolic fractions, the cells were fractionated in advance as described below.

The beads were subjected to four rounds of washing with 1 ml of lysis buffer, then immunoprecipitates were eluted by adding electrophoresis sample buffer.

Beads were then washed three times with 1 × lysis buffer, and immunoprecipitates were eluted with SDS gel loading buffer (Tris-HCl 63 mM, SDS 1%, phenol red 0.03%, glycerol 10% and DTT 100 mM of pH6.8), boiled for 5 min and processed for immunoblotting.

Beads were washed with lysis buffer and immunoprecipitates were eluted by boiling with Laemmli sample buffer.

After incubation for 2 hours at 4°C, the beads were washed three times with the lysis buffer, and the immunoprecipitates were eluted using 3× Laemmli sample buffer and were subjected to SDS-PAGE and western blotting using indicated antibodies.

Finally, the immunoprecipitates were eluted with elution buffer (sample-loading buffer in 50 m m Tris HCl, pH 8.0) by heating for 10 min at 95°C and analyzed by western blotting and by ISIL followed by in-gel digestion for the determination of their Nt-acetylation states (62).

The beads were washed four times with buffer A containing 0.5% NP-40 and 10% glycerol and the immunoprecipitates were eluted from the beads with Laemmli buffer at 95°C for 5 min.

Sepharose beads were washed twice in lysis buffer by centrifugation at 14,000 r.p.m. for 2 min at 4°C and, finally, immunoprecipitates were eluted by boiling in SDS-sample buffer.

Immunoprecipitates were eluted in 2× sample buffer.