The tissue blocks were immediately fixed in 10% buffered formalin for one week and processed into paraffin blocks.
The other half was fixed flat in 10% buffered formalin (Fisher Scientific, Fair Lawn, NJ) between two filter papers for one week before Aberrant Cypt Foci (ACF) enumeration.
There was a positive correlation between wind speed for two weeks prior in Shandong Province counties within the 50-km buffer circle and wind speed for one week prior in those counties.
Excess radioactive probe was washed out with 2x SSC, 0.1% SDS buffer, and the blots were exposed for one week to phosphorimaging screen (Fujifilm) and scanned with Fujifilm FLA5100 reader (Fujifilm Co., Ltd., Tokyo, Japan).
To explain the rationality of correlating meteorological factors within two weeks prior with the incidence, we performed SVD analysis of wind speed for two weeks prior in the counties within the 50-km buffer of Shandong Province and wind speed for one week prior in these counties.
(1) SVD analysis of wind speed for two weeks prior in counties within the 50-km buffer circle of Shandong Province and wind speed for one week prior in Shandong counties.
Figures 5(a) and 5(b) display the first pair of SVD analysis modes between wind speed for two weeks prior in the counties within the 50-km buffer circle of Shandong Province and wind speed for one week prior in the these counties.
Thus, the variance of the first pair of singular vectors was large, indicative of the coupling relationship between wind speed for two weeks prior in the counties within the 50-km buffer circle of the province and wind speed for one week prior in these counties.
Figures 5(a) and 5(b) show a significant positive correlation between wind speed for two weeks prior in the counties within the of 50-km buffer circle of the province (left field) and wind speed for one week prior in these counties (right field).
That is, when wind speed for two weeks prior in the counties within the 50-km buffer circle in the northwest province was anomalously small (large), wind speed for one week prior in the Shandong counties was abnormally small (large).
