Sentence examples for buffer for one minute from inspiring English sources

24 hours post-transfection cells were washed and permeabilised in the following ways: Selective permeabilisation of the plasma membrane was obtained by treating cells with 40 µM digitonin in KHM buffer for one minute before fixation using 4% formaldehyde in PBS.

After removal from the hybridization station the microarray slides were sequentially washed in post wash buffer and isopropanol for one minute before being dried by centrifugation at 220 g for 6 minutes.

After removal from the hybridization station the microarray slides were sequentially washed in post-wash buffer and isopropanol for one minute before being dried by centrifugation at 220  g for six minutes.

After having removed the aliquots, the pellets were dyed with 200 μl (1.44 ml/100 ml Hank's buffer) acridine orange (Sigma, A6014-10G) foneone minute.

Sponges from the hand and environmental swabs were transferred aseptically to homogenizer bags into which 20 mL of Butterfields phosphate buffer was added and squeezed for one minute by using a hand roller homogenizer.

After incubating the mixture at 60°C for 15 minutes, it was immediately cooled on ice for one minute before adding an equal volume of 2X GEx hybridization buffer HI-RPM (Agilent Technologies, Mississauga, ON, Canada).

The bound DNA was eluted with 80 μL buffer AE, incubated at room temperature for one minute and centrifuged at 8,000 rpm for one minute.

Arrays were washed for one minute with Gene Expression Wash Buffer I at room temperature, and one minute with Gene Expression Wash Buffer II at 37°C.

Tissue fragments were diluted (1 10) in the same buffer, homogenized (three cycles at 350 g for one minute) and centrifuged as above.

This was followed by holding the arrays in nonstringent hold buffer until ready for wash in 0.2× SSC for one minute followed by 30 seconds wash in 0.05× SSC.

The tube was inverted so the buffer covers the cap, vortexed at a medium speed for one minute and incubated at room temperature for 5 minutes.