Sentence examples for buffer for one hour from inspiring English sources

The tissue was incubated in the soluble extraction buffer for one hour on a rocking platform at 4°C.

Incubation of the eluted LVs in the high salt buffer for one hour on ice resulted in a significant reduction of infectivity by 16.0 ± 6.4%.

They were then rinsed three times with washing buffer, 0.1% Triton X-100 in PBS before being incubated with Alexa Fluor 488 Goat anti Rabbit secondary antibody (Invitrogen Molecular Probes, Oregon, USA) at a dilution of 1 1000 in blocking buffer for one hour at room temperature.

The eye-cups were then dissected and fixed in 4% paraformaldehyde in a phosphate buffer for one hour.

Cells were then washed and postfixed in 1% osmium tetroxide with 1.5% potassium ferrocyanide in the same buffer for one hour.

The membranes were washed twice with TBST and incubated with Peroxidase from horseradish (HRP) conjugated secondary antibody (Cell Signaling, Boston, MA, USA) in blocking buffer for one hour.

Ellipsometry measurements demonstrated only a 0.2 nm reduction in the thickness of an 18 nm thick ppAAc coating, when immersed in an aqueous buffer solution for one hour.

In each case, cells grown to about 70% confluency were scraped, pelleted by centifugation, fixed in 10% buffered formalin for one hour, stored in 70% ethanol and ultimately paraffin embedded.

Mouse anti-human Id1 antibody (Abcam) in blocking buffer was added for one hour.

Samples were diluted in assay buffer and incubated for one hour, followed by incubation for one hour with tracer antibody.

Samples were fragmented using kit-provided reagents and buffer at 37°C for one hour and precipitated by adding 2-propanol.