Sentence examples for buffer for one h from inspiring English sources

A 200 +/− 25 bp band was excised from a 2% agarose gel after electrophoresis in 1× TAE buffer for one h at 120 V.

Cells were seeded in 12-well plates and incubated for 36 48 h before incubation for an additional 16 h with 2 mM glucose in DMEM with 0.1% BSA, followed by 2 mM glucose in KRB buffer for one h.

PEG-purified phage in blocking buffer (1 1) was added to each well and incubated for one h at 37°C.

After discarding coating solution, plates were blocked for one h at 37°C with blocking buffer (5% milk, 0.05% Tween 20 in PBS).

The membranes were blocked for 2 h in blocking buffer (Odyssey blocking buffer and PBS, 1 1 with Tween 0.05% v/v) and incubated with patient sera in a dilution of 1 10 in blocking buffer for one hour.

Cells were permeabilized by washing 3 × 5 min with PBS, 0.1% Triton X100, followed by blocking in 5% nonfat dry milk in PBS, 0.1% Triton X100 for 1 h at RT. Cells were incubated with 50 μl primary antibody (anti-p27, 1 1000 in blocking buffer) for 1 h, followed by one wash with blocking buffer.

After blocking and washes, samples were incubated in the SUMO assay buffer for 1 h.

Samples were then incubated in hybridization (Hybe) buffer for 2 h at 65 °C.

Slides were then submerged into cold lysis buffer for 3 h.

The membrane was blocked with 3% BSA in TBST buffer for 2 h.

Cell pellets were treated with 2% osmium tetroxide in 0.2 M cacodylate buffer for 2 h at 4 °C, and rinsed in 0.1 M cacodylate buffer.