Sentence examples for buffer for determination of from inspiring English sources

We used six volumes of lysis buffer for determination of IFN-α in liver samples and eight volumes of buffer for determination of IFN-γ.

After incubation the culture media were removed, the cell layers were washed with PBS and submitted to the action of lysis buffer for determination of ORP150/GRP170 expression and protein assay.

Serum samples were diluted in casein buffer 1 500 for determination of IgG1, IgG2a, and IgG2b, and 1 20 for IgA and IgE.

The enzymes (PLA2S) to be examined were dissolved in 1 M Tris-HCl buffer (pH 7.4) for determination of the activities (MIC).

For quantification of mitochondrial protein, an aliquot was removed, centrifuged at 8,000 g for 15 min, washed in BSA-free isolation buffer, pelleted, and used for determination of protein concentration.

All incubations were done at room temperature, and all washings were performed with phosphate-buffered saline buffer (pH 7.5) Control sections used for determination of antibody reaction specificity: (a) tissue positive control (sections of mouse spleen) and (b) negative technique control (serial sections of each sample omitting the primary antibody).

Aliquots of the resultant supernatants were then either acidified with metaphosphoric acid to a final concentration of 5% (wt/vol) for AA and urate measurement or diluted into 100 mM phosphate buffer for the determination of reduced GSH.

At the end of the incubation, the media were collected for insulin measurement and the cells recovered in acidified ethanol (0.2 mM HCl in 75% ethanol) or in lysis buffer for the determination of cellular insulin or protein content, respectively.

Reaction vessels containing only the virus and buffer without chlorine served as controls for determination of virus titers in the absence of chlorine and were assayed at the end of the longest exposure time period (60 s).

Britton Robinson buffer (pH 2.0) was chosen as optimal electrolyte for determination of triclopyr using square wave and differential pulse voltammetry.

In general, rhPADs were diluted in citrullination buffer and incubated for 3 hours at room temperature for determination of activity and calcium dependency.