Sentence examples for buffer for analysis of from inspiring English sources

When [14C]AA was used as substrate, the dried extracts were re-dissolved in acetonitrile for analysis of representative PG products by HPLC, and when PGG2 was used as substrate, the dried extracts were re-dissolved in the EIA buffer for analysis of PGE2 using an EIA kit.

BXD25 and BXD40 mouse brains were lysed directly in radioimmunoprecipitation (RIPA) buffer for analysis of whole cell lysates.

Briefly, ~2 × 10 PBMCs were incubated with APC-conjugated anti-CD5/FITC-conjugated anti-CD19 or isotypes (BD, USA) for 20 min at 4°C, washed twice, and resuspended in staining buffer for analysis of B cell subpopulations.

One fifth of suspension was centrifuged for 1 min at 10 000 g, supernatant was removed and SP cells were lysed in SDS sample buffer for analysis of total translation products.

Mixtures were incubated at 68° for up to 80 hr and samples were periodically removed into 10 mM phosphate buffer for analysis of secondary structure by elution from hydroxyapatite (Biorad).

~2 × 10 PBMCs were incubated with FITC-conjugated anti-CD4/PE-Cy7-conjugated anti-CD25/APC-conjugated anti-CD127, or isotypes (BD, USA) for 30 min at 4°C, washed twice and resuspended in staining buffer for analysis of T cell subpopulations.

Finally, the cell pellets were resuspended in 200  μl of buffer for analysis on a FACSCalibur flow cytometer (Becton Dickinson).

After reaction, remove 1 μl and add it to 3 μl 2X Laemmli buffer for analysis to check for expression of the protein.

The SEC buffer used for analysis of the HsAPC-1 RD protein was purification buffer with either 5 mM calcium or 5 mM EGTA, with or without 1 mM DTT added as stated.

After 24 hours, the cells were washed with Dulbecco PBS and lysed using Buffer RLT for analysis of TLR mRNA by quantitative PCR.

The SEC buffer used for analysis of the full-length HsAPC-1 was 20 mM Tris pH 7.4, 50 mM NaCl, 0.03% (w/v) LMNG and 0.03 mg mL− 1 tetra-oleoyl cardiolipin.