Sentence examples for buffer for a total from inspiring English sources

Each site received 250 nl of hCNS-SCns or hFb or vehicle (injection buffer), for a total volume of 1 µl, delivered in 50 nl puffs over 75 s, followed by a 2 min delay before withdrawal of the pipette.

Wells were then vacuumed and washed with 100 μl of resuspension buffer for a total of three washes.

Four tubes were prepared with that contained 80 μM phosphorylated HS-Fc-P, 30 μM MB-P, 30 μM Ligation-P (see also Scheme 1), and 1× ligation buffer for a total of 100 μL per tube.

Each 5-μL oocyte sample in RLT buffer was mixed directly with 10 μL nCounter reporter probes, 5 μL nCounter capture probes, and 10 μL hybridization buffer for a total reaction volume of 30 μL.

The animals were then rinsed five times on ice using the same buffer for a total of 5 min. Post-fixation was carried out with 1% OsO4 in Sorensen's phosphate buffer on ice.

Samples from each tree were thawed upside down on ice and contents were pooled into a 1.5 ml microfuge tube and the PCR tube caps were each washed with 20 μl protein extraction buffer for a total volume of 200 μl.

The cells were permeabilized by adding 50 μl dichloromethane, 20 μl 0.1% SDS, 5 50 μl resuspended cells (out of 150 μl) plus Z-buffer for a total of 150 μl including cells, followed by vortexing for 10 s.

Ternary complexes were eluted with 600 µl elution buffer (TL + 250 mM imidazole) and dialyzed into Buffer TL for a total of 4 hr.

Therefore, data for these economic variables were gathered from separate studies of 26 Brazil nut concessions located outside of the TNR, BSNP and buffer zone, for a total of 27 concessions (Supporting Information Table S5).

Sections were then rinsed six times in tris buffer saline for a total time of 90 min.

In order to unmask the antigens, the slides were microwave treated in 10 m M citrate buffer, pH6, for a total of 10 min.