Sentence examples for buffer for a period from inspiring English sources

The enzyme was incubated at temperature ranged 20°C to 50°C in 100 mM pH 6.0 Na-acetate buffer for a period of time and residual activity was assayed in optimal temperature and pH by DNS method.

The pellet was resuspended in PBS, pH 7.4 and dialyzed exhaustively against the same buffer for a period of 48 h.

Fluorescein isothiocyanate-conjugated EGFR antibody was added to the cells resuspended in 100 μl FACS buffer for a period of 1 hour in the dark at 4°C.

The entire ventricle was cut from the apex to base into four transverse slices and incubated in 2,3,5-triphenyltetrazolium chloride (TTC) (10 mg/mL in phosphate buffer) for a period of 10 min at 37°C to visualize the infarct area.

Upon completion of the ligation reaction the ligation mixture was eluted from the chromatography column with 15 ml of dialysis buffer (50 mM Tris pH 8.0, 150 mM NaCl, 10 mM DTT) and subsequently dialyzed into 4 l of dialysis buffer for a period of 48 hr with multiple buffer exchanges in a dialysis cassette (Slidealyzer) with a 12K MWCO in order to remove excess unreacted peptide.

The enzyme was incubated at 20°C in pH 5.0 to 7.0 100 mM buffers for a period of time.

In keeping with earlier studies using the canine ventricular wedge preparation, we perfused all murine hearts with buffer solution for a period of 30 min in order to washout residual catecholamines that may be leaking from nerve terminals (Krishnan & Antzelevitch 1991).

After flowing buffer for a short period, a second injection of DR1 pre-incubated with either SEH or SEA at a 1∶1 molar stoichiometry was performed.

Following stratification, subjects will be randomized centrally to receive either the lactate-containing Balance solution or the bicarbonate buffered Bicavera® solution for a period of 10 months.

Samples were injected for 3 s and then electrophoresed using 3730 buffer with EDTA (ABS) for a period of 5000 s at a voltage of 15 kV and a current of 400 mA.

The devices can buffer traffic for a certain period if the destination devices stay in doze mode, and during ATIM (announcement traffic indication message) window, all the devices become active for ATIM frame transmissions.