Sentence examples for buffer for a minimum from inspiring English sources

Detergent was removed by incubating the mixture with prewashed Amberlite-XAD beads that were prewashed with methanol and resuspended in buffer for a minimum of 2 h at 25 °C, and the mixture was passed several times through a 200 nm PC membrane using an extruder.

This tissue was then fixed in 10% buffered formalin for a minimum of 2 weeks to allow for sufficient fixation.

Tumors were fixed in 10% buffered formalin for a minimum of 24 hours, paraffin-embedded and sectioned for H & E staining.

A sample (max volume = 10 × 10 × 5 mm) of pyloric caeca and surrounding tissues (designated as the injection site) and head kidney were collected from each fish and stored in 10% phosphate buffered formalin for a minimum of 4 days.

Flat-mounted retinae were post-fixed in fresh 2.5% glutaraldehyde in 0.1 M sodium cacodylate buffer at 4°C for a minimum of 12 hrs and then further fixed in 1% osmium tetroxide.

For polarized-light microscopy, internode segments were fixed in 2% glutaraldehyde in 50 mM Na2PO4 buffer (pH 7.2) for a minimum of 2 h at room temperature and then handled and embedded in Spurr's resin by standard techniques.

Then, they were digested using a solution containing 5 mg/ml collagenase, 2.4 U/ml dispase and 0.05% trypsin in phosphate buffer saline (PBS) for a minimum of 1 hour.

For histology, intact mouse knees were dissected away from the skin, fixed with 10% neutral buffered formalin for a minimum of three days, and decalcified in 5% ethylenediaminetetraacetic acid (EDTA)/phosphate-buffered saline (PBS) for three weeks.

During histopathological analysis, prostatectomy specimens were fixed in 10% buffered neutral formalin for a minimum of 24 hours.

After removal from the implantation site, each graft was placed in 10% buffered formalin solution for a minimum of seven days.

The drug release occurred at a faster rate in phosphate buffer solution and continued for a minimum period of 8 h.