Sentence examples for buffer for a final from inspiring English sources

A final dilution to 8 to 12 pM was performed with HT1 buffer for a final volume of 1 ml.

For this experiment, 153 nt ssDNA was added to the previously described cis chamber buffer for a final concentration of 8 nM.

Following addition of 400 μl binding buffer for a final volume of 500 μl the cells were stained with PI (0.6 μg/ml) immediately before measurement.

Hybridization cocktails contained 15 μg fragmented cRNA, 5 μl 3 nM control oligonucleotide B2, 15 μl 20X eukaryotic hybridization controls, BSA (0.5 mg/ml), herring sperm DNA (0.1 mg/ml), dimethyl sulfoxide (DMSO) (10%), and hybridization buffer for a final volume of 300 μl.

The SUVs (composition as above) were diluted in the same buffer for a final concentration of 150 μg/ml; 500 μl of this dilution was applied at a flow-rate of 50 μl/min and 28°C to form the SLB [ 20].

After an incubation time of 10 min at room temperature, additional 400 μl of binding buffer were added for a final volume of 500 μl.

The reaction mixture consisted of: 100 mmol/L potassium phosphate buffer (pH 8) for a final concentration of, 0.1 mg/mL BSA and 50  μL of cell homogenate for a total of 15  μg of protein.

To study the separation and detection of the bacteria from the blood cells, a 1× phosphate-buffered saline (PBS) buffer diluted with the 300-mM sucrose solution in a 1 15 ratio was used for the experimental buffer with a final conductivity of 1 mS/cm, owing to the fact that blood cells are highly sensitive to the osmotic pressure of a solution.

The reaction was then quenched for 15 min adding Tris buffer at a final concentration of 50 mM.

For initial velocity measurements (v0), aliquots of the reaction were quenched at six time points between 15 s and 10 min. The reactions were quenched by the addition of 5× quench buffer (250 mM EDTA) for a final concentration of 50 mM EDTA.

For initial velocity measurements, aliquots of the reaction were quenched at six time points between 15 s and 10 min. The reactions were quenched by the addition of 5× quench buffer (250 mM EDTA) for a final concentration of 50 mM EDTA.