Sentence examples for buffer exchange with a from inspiring English sources

Following buffer exchange with a Hepes buffer to eliminate reducing agent, 10mM of the Hg compound CMNP (6-chloromercuri 2,4-dinitrophenol-Kodak) was added to the protein and incubated for 4 hours.

PDI was reduced for 1 h with 10 mm DTT and then buffer exchanged with a PD10 column into 50 mM Tris HCl buffer, pH 7.5.

All PhoP samples, with or without a His tag, were further purified and buffer exchanged with a Superdex 200 column (GE Life Sciences) for downstream applications.

Samples were buffer exchanged with an Amicon Ultra centrifugal filter (0.5 mL, 10 kDa) to a buffer containing 50 mM NaH2PO4 and 50 mM sodium acetate (pH 3) and incubated for 1 h at 37 °C.

The purified enzymes eluted by the high concentration of imidazole were subjected to buffer exchange with buffer A by YM-3 Microcon device (Millipore).

The purified recombinant protein was obtained with a stepwise combination of Ni-NTA affinity chromatography, desalting and buffer exchange with Zeba desalting columns [ 25].

Eluates were concentrated and subjected to buffer exchange with phosphate buffer pH 7.4 using Amicon ultra 10,000 MWCO centrifugal filter devices (Millipore).

Eluate was concentrated and buffer exchanged with PBS on a Amicon Ultra-4 10K MWCO spin column (Millipore, Billerica, MA) and purified scFv concentration determined as described above.

Mature human recombinant OSM, fused to a 6xHis tag at the amino terminus, was expressed in E. coli, purified by nickel columns, and buffer exchanged with phosphate buffered saline (PBS) (see Materials and Methods for details).

All TTR samples were buffer exchanged with complete growth medium just before the treatment and filter sterilized.

The FtsY-Hg complex was then buffer exchanged with Hepes buffer using Centricon YM-10K (Amicon) to eliminate unbound Hg.