Sentence examples for buffer exchange was performed from inspiring English sources
The eluted protein was concentrated, and a buffer exchange was performed with 25 mM phosphate buffer containing 10 µM FAD.
Finally, the protein was concentrated and the elution peptides removed using a centrifugal concentrator with 10,000 molecular weight cut off, Vivaspin 6 (Sartorius Stedim Biotech SA, Aubagne Cedex, France) and buffer exchange was performed 10 times to remove the peptide.
Buffer exchange was performed for three times by diluting with 10 vol of phosphate buffered saline (PBS) and concentrating in an Amicon Ultra spin filter.
Buffer exchange was performed to 0.1 M MES pH 5 using Amicon Ultracel 3K 4 ml (Millipore) to provide optimum reaction conditions for biotinylation.
One mL of each sample was desalted through a 10 DG column (Bio-Rad, USA) and buffer exchange was performed with 10 mM Tris.Cl, pH 6.8.
MBP-tagged PPLP2RP1 and PPLP2RP2 peptides were purified via affinity chromatography as described above and PBS buffer exchange was performed via filter centrifugation with Amicon Ultra 15 centrifugal filter units (Sigma Aldrich) according to manufacturer's protocol.
Three buffer exchanges were performed with a spin filter device of 5,000 Da molecular weight cut-off to reduce salt concentration before storing at −80°C until further use.
