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Buffer exchange was done three times by using ultracentrifugation (50,000 rpm for 20 min at 4 °C), with resuspension of the protein in the appropriate buffer and equilibration for 30 min at ambient temperature after each exchange.
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For GEF reactions, the nucleotide exchange is done in the presence of GEF proteins and does not require a low-Mg2+ buffer.
After cleavage, a buffer exchange was performed to remove DTT prior to the second purification step.
Buffer exchange was repeated once.
The eluted protein was concentrated, and a buffer exchange was performed with 25 mM phosphate buffer containing 10 µM FAD.
Imaging was initiated as soon as the buffer exchange was complete.
Options exchanges are doing a booming business.
The exchanged buffer exchanged was 0.01 × PBS/0.01 mM DTT/2.5 mM imidazole/0.1% glycerol.
Buffer exchange to remove imidazole or desthiobiotin was done using Vivaspin centrifugal concentrators (Sartorius Stedim, Göttingen, Germany).
In some cases data is copied out of the buffer to a second buffer; this is done using functions ('parse_str', 'parse_attr' and 'strncpy that take a buffer-length argument, so again there can be no buffer overruns.
He was doing a 1031 tax exchange.
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