Sentence examples for buffer exchange in a from inspiring English sources

HPTFF takes into account both the size and charge of a molecule and can perform protein concentration, purification, and buffer exchange in a single operation, hence reducing the cost of downstream processing [ 127].

High-performance tangential flow filtration can also perform concentration and buffer exchange in a single step (Section 4) and this methodology appears to be gradually replacing SEC in industrial downstream processing.

EPR samples were prepared by buffer exchange in a centrifugal concentrator.

For soluble protein samples, spin-labeled proteins were buffer exchanged in a 10 kDa molecular weight cutoff protein concentrator (Millipore) to ~80% deuterium buffer of 150 mM NaCl, 20 mM HEPES pD7.4 supplemented with 30% (v/v) glycerol-d8.

The protein was then concentrated and buffer exchanged in a 3 kDa molecular weight cutoff Amicon Ultra (Millipore No. UFC900324) into 20 mM Tris-HCl pH 7.6 to a final concentration of 50 100 mg mL 1.

In brief, products were subjected to ethyl acetate extraction (1 1) by vigorous shaking for 30 min. The separated aqueous layer was then immediately treated to buffer exchange in PBS using a Centriprep centrifugal filter device (Millipore, Bedford, MA) to remove trace residual ethyl acetate.

In a second approach nickel affinity purified PsOep23 was subjected to buffer exchange in 20 mM sodium phosphate pH 7.0, 8 M urea using Amicon centrifugal filters (MWCO 10 kDa, Merck).

Antibodies were purified on a protein A column (GE Healthcare), eluted with 50 mM citrate buffer, pH 3.5, and buffer exchange in 20 mM His, pH 6.5 buffer for stability analysis or 50 mM Tris buffer for fluorescence labeling.

AAV5 GFP vector-containing fraction was then concentrated and buffer exchanged in Alcon BSS with 0.014% Tween 20, using a Biomax 100 K concentrator (Millipore, Billerica, MA, USA).

Protein samples were buffer exchanged in 49.5/49.5% of H2O/CH3OH and 1% of formic acid using an Amicon centrifugal filter device, 3 or 10 kDa cutoff (Millipore).

To obtain completely unfolded TTR, the proteins were first incubated in 6.5 M GndCl for 24 h and then buffer exchanged in 9 M urea as described previously.