Sentence examples for buffer embryos were incubated from inspiring English sources

After washing again with PBTx and incubation for 1 hour in block buffer, embryos were incubated in the second antibody overnight at 4°C.

After 3 times washing with acid alcohol buffer, embryos were incubated for 10 min with bleaching buffer (1% H2O2 and 1% KOH).

After washing (3 × 10 minutes) with blocking buffer, embryos were incubated with the secondary antibody (Fluorescein (DTAF -conjugated AffiniPure Goat anti-Mouse IgG, Jackson Immuno Research Laboratories, Inc., 1:100) for 1 hr.

Primary antibody was washed off by ABS buffer, and embryos were incubated overnight with secondary antibody goat anti-mouse conjugated with Alexa-488 (1∶250, Molecular Probe)[Tucker et al, 2006].

For C. albipunctata embryos, the following modifications to the staining protocol apply: proteinase K treatment was carried out for 7 min at room temperature; post-hybridization washes: an additional wash of 10 min 2xSSC/hybridization buffer was performed before washing for 15 min with PBT/hybridization buffer; antibody incubation: embryos were incubated with anti-DIG for 2 hrs.

After thorough washing with buffer B, the embryos were incubated in Hoechst 33258 stain before mounting for confocal microscopy.

After washing in a serial of wash solution buffers and treating with ribonucleases, the embryos were incubated with anti-digoxigenin alkaline phosphatase (AP -conjugated AP -conjugatede) overnight with rocking antibody

After post-hybridization washes, embryos were incubated in blocking buffer (PBS/Triton 0.1% containing 15% serum and 2 mg/ml BSA) for 3 hours at 4°C before addition of the alkaline phosphatase coupled anti-digoxygenin antibody (1∶1500, Roche) for 12 hours at 4°C.

To allow embryos to develop to later stages, collected embryos were incubated in M9 buffer at 20°C for 5 hours.

Embryos were incubated in transplantation buffer [0.5× MMR, 1% ficoll and 5 μg/ml gentamicin (Sigma)] throughout the transplantation procedure.

Control embryos were incubated in Hanks' buffer solution instead of primary antibody and then treated with complement.