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A mixed-stage worm culture was washed of the plates with M9 buffer, eggs were prepared by hypochlorite treatment and were allowed to hatch over night in M9 buffer.
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Mouse sperm and eggs were prepared as above.
Toxocara canis eggs were obtained from adult female worms, and embryonated eggs were prepared as previously described [ 22].
Three different buffer solutions were prepared: pH 4 citrate buffer 0.1 M, pH 6 citrate buffer 0.1 M and PBS 1x (pH 7.4).
Fresh buffer and standards were prepared daily.
Xenopus egg extracts were prepared essentially as described [10].
Interphase egg extracts were prepared as previously described [45].
All media and buffers were prepared essentially as described [29].
All buffers were prepared from reagents of standard grade.
Stage 14 egg chambers were prepared as above.
All buffers were prepared with deionized water.
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