Sentence examples for buffer denatured by the from inspiring English sources

Beads were washed three times with lysis buffer, denatured by the addition of 50 μl sample buffer, and analyzed by Western blot.

Equal amounts of total cell lysates (20 60  μg) were mixed with Laemmli buffer, denatured by boiling, and separated by sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) as we previously described [ 37].

Immunoprecipitates were washed 3 times with 0.3% CHAPS lysis buffer and denatured by the addition of 20  μl 1 × SDS sample buffer and boiling for 5 min.

The 2M eluate was dialyzed against buffer D, denatured by the addition of solid urea to a final concentration of 6M and loaded onto a 1×5 cm Poros 20 HQ column equilibrated in buffer D-6M urea.

The beads were washed in the same IP buffer and finally denatured by the addition of Laemmli buffer.

Pull-down beads were washed 5× with RIPA lysis buffer and proteins were denatured by the addition of Laemmli sample buffer (Biorad) supplemented with 5% β-mercaptoethanol.

The precipitated proteins were then washed five times with RIPA buffer, and denatured by heating the beads in sample buffer at 95°C for 5 min. Samples were analyzed by SDS-PAGE (5 20% gel, BioRad, Tokyo, Japan), and transferred onto a PVDF membrane (GE Healthcare Biosciences, Buckinghamshire, UK).

An equal volume (50 mg) of samples was denatured by the addition of sample buffer (Bio-Rad Landratories, Munich, Germany) and boiled for 4 min. Proteins were resolved under reductive conditions with SDS-PAGE and transferred onto PVDF membrane (Life Technologies, Gaithersburg, MD, USA).

The samples were finally denatured by the addition of Laemmli buffer.

The template solution was denatured by the addition of 3.5 µl buffer DLB for 5 minutes at room temperature.

Antibody was denatured by the addition of 1× SDS sample buffer + DTT and heating at 95°C for 5 min.