The cells were washed with DMEM-F12, and then with staining buffer, counted and finally resuspended in FACS staining buffer (PBS with 3% FBS and 0.09% sodium azide).
Protoplast was filtered through nylon and washed by W5 buffer, and re-suspended in MMg Buffer, counted under the microscope, then adjusted at final concentration of about 1 × 10 mL-1.
The supernatant was removed and the coupled microspheres were resuspended in 500 μL of storage buffer, vortexed, removed, and finally resuspended in 150 μL of storage buffer, counted, and stored between 2 and 8°C in the dark.
P = Counts perminin octanole – Counts perminback ground Counts perminin buffer – Counts perminback ground (1).
For one of each control and experiment plate, we collected all of the fruiting bodies in 1 ml of KK2 buffer to count the number of spores that were produced.
Prior to use in these studies, yeast were grown in YPD liquid medium overnight at 30°C, harvested, washed three times with sterile phosphate-buffered saline (PBS), suspended in phagocytosis buffer and counted with a hemacytometer to determine cell concentrations.
In MDC-SR, they define an upper bound threshold for the number of buffered bundles such that if a node holds more buffered message count than an upper bound, the drop procedure will not be called.
The decision by the 27 bodies that make up the Basel committee to give banks a further four years to build up their liquidity buffers – and count more types of assets as "liquid" – comes after months of lobbying.
Nuclei were resuspended in 60 μl of PHEM buffer and counted using a hemocytometer.
Cells were washed and resuspended in flow buffer before counting and staining for flow cytometry.
