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Equal numbers (n=9) of mice were injected with buffer control in a paired design.
Each sample lysate was prepared in 5 dilutions, neat plus 4 serial dilutions with a 0.8 dilution factor, and 1 buffer control, in a 384-well V-shaped ABgene plate (Thermo Fisher Scientific, Rockford, IL).
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Briefly, 100 μl plasma samples were applied to microtitre wells coated with CEA or phosphate buffer (control) in duplicate and incubated for 1 h at room temperature on a plate shaker.
The buffer control in the absence of analyte is shown as black symbols.
Graphs show the percentage of embryos in each class for RNAi and the buffer control in that experiment.
While administration of CpG caused a substantial increase in spleen size, the protamine/ mRNA-complexes were indistinguishable from buffer controls in this respect which already indicated that mRNA based vaccines might exhibit a very good safety profile.
Although for the initial ee values in the buffer controls in the range of 50% (S) to 30% (R) significant ee shifts were observed, they correlate with only very small changes of the free energy of activation.
Regarding the rpd values for the buffer controls in comparison to each other, taking into account the reduction of the rpd values in the vertical direction, it seems that the production of acetoin (rdp=0.69) is more resistant to the addition of organic solvents than the production of PAC (rpd=0.3) and benzoin (rpd<0.01).
Collected biopsies (N=3 6) were incubated with 250 μL of carbogenated solutions of buffer (control) or nutrient in a 96-well plate for 15 min (5-HT assay) or 2 h (GLP-1 and PYY assay) at 37°C.
Repair studies were performed by irradiating preembedded cells on crushed ice; slides were then placed in ice-cold lysis buffer (control) or in growth medium at 37°C in 95% air: 5% CO2 for specified times prior to lysis.
No production occurred in the buffer control or in reactions lacking NADPH.
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