Sentence examples for buffer containing the protease from inspiring English sources

The tissue samples were snap frozen with liquid nitrogen, ground into powder, and lysed on ice for 30 min in RIPA lysis buffer containing the Protease and Phosphatase Inhibitor Cocktail.

HUVECs in 10-cm dishes were lysed in 200 uL Chaps buffer containing the protease inhibitors and PMSF.

The membrane pellet was washed in 800 μl homogenization buffer containing the protease inhibitors indicated above and centrifuged at 117,000  g at 4 °C for 1 h.

Cells were collected and lysed by scraping into RIPA buffer containing the protease inhibitors phenylmethylsulfonyl fluoride (1 mM), sodium orthovanadate (1 mM) and aprotinin (60 μg/ml).

Cells and tissues were lysed in RIPA lysis buffer containing the protease inhibitors phenylmethylsulphonylfluoride (PMSF) (1 mol/l) and EDTA (1 mol/l), Na3VO4 (1 mol/l), and NaF (1 mol/l).

Cancer tissues and adjacent normal tissues from all 63 patients were homogenized in radioimmunoprecipitation assay buffer containing the protease inhibitors phenylmethylsulfonyl fluoride (100 μg/mL), NaVO3 (1 mmol/L) and dithiothreitol (0.5 mmol/L).

All subsequent procedures were performed on ice, with buffers containing the protease inhibitors (cOmplete EDTA-free) as previously described [21].

Cultured cells at subconfluent healthy state were collected in ice-cold PBS containing 1 m M PMSF, 2 m M sodium orthovanadate (Sigma Co., St Louis, MO, USA), 2 m M NaF, and complete protease inhibitor cocktail (Boehringer Mannheim, Germany) and then were lysed in modified radioimmunoprecipitation (RIPA) buffer containing the above protease and phosphatease inhibitors.

Samples were homogenized using an Ultraturax homogenizer in lysis buffer containing the provided protease inhibitors.

Cell lysates were prepared with RIPA buffer containing the complete protease mix (Roche).

Tumour powders were dissolved in lysis buffer containing the Complete Mini anti-protease cocktail (Roche) and incubated for 2 h at 4°C.