Sentence examples for buffer conditions were used from inspiring English sources
We were still unable to identify Polo interacting with Feo::PtA when alternative buffer conditions were used and Polo was over-expressed (data not shown).
The binding buffer and conditions were used as in [ 17], but 0.1 mg/ml buffer of heterologous herring sperma DNA was added.
Equal amounts of protein were diluted with sodium dodecyl sulfate (SDS) sample buffer, (reducing conditions were used only for p21) boiled for 5 min, and electrophoresis performed using SDS-polyacrylamide gel electrophoresis (SDS-PAGE).
Standard buffers and conditions were used for electrophoresis and immunoblotting.
Capsule-inducing conditions were used.
Therefore, we sought to analyse the affinities of DdGAB and hGAB peptides for G-actin under buffer conditions that were used in the TIRF experiments.
The G-quadruplex structures and stabilities were characterized by CD spectroscopy to demonstrate that the 22-nucleotide sequence (Tel-22) was folded into G-quadruplex structures under the Na+ and K+ buffer conditions that were used and exhibited thermal stabilities and folding topologies comparable to those previously published.
In this search it has been noted that the buffer conditions that are used for HILIC are highly compatible with MS, and that the high organic contents of these buffers can potentially increase sensitivity in ESI MS[ 9, 10].
Some modifications in assay conditions (Buffered Method) were used to control potential variables across the experiment (including effects of brain pH and other brain components on the reaction).
For morphological analysis animals were perfusion fixed under thiopental anesthesia (12 mg/100 g body weight, intraperitoneally; either on day 15 or day 85 after the first PSI/vehicle injection) with 10 ml phosphate buffered saline (PBS; 25 mM, 0.9 % NaCl, pH 7.4) followed by chilled fixative in PBS (buffer conditions are given below for the different techniques used).
Animals were deeply anesthetized by intraperitoneal injection of thiopental (12 mg/100 g body weight) and perfused transcardially with phosphate-buffered saline (PBS; 25 mM, 0.9 % NaCl, pH 7.4) followed by chilled fixative (respective buffer conditions are given beneath for the different techniques used).
