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The range of optimal buffer compositions that maximized thermostability and minimized viscosity of a mAb formulation was determined.
The effect of pH upon the potentiometric response was evaluated for different pHs (2-9) with different buffer compositions.
Then the key refolding buffer compositions were selected for further optimization by means of the Box Behnken design of response surface methodology (RS M.
The optimization of buffer compositions was guided by statistical analysis of the data to obtain the targeted combination of low viscosity and high thermostability.
Some surface plasmon resonance (SPR) biosensors allow for parallel screening of several running buffer compositions, with a number of different immobilization levels for each buffer.
In the first stage of study several release media containing different buffer compositions, pH, and a series of micelle forming PEO PPO PEO block copolymers (known as Pluronic®) were examined for solubility and stability of SRL by reversed phase high performance liquid chromatography (RP-HPLC).
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Buffer composition and voltage were optimised using a central composite design.
Separation parameters such as background buffer composition, voltage, temperature, and nebulizer pressure were optimized using a multivariate central composite design (CCD).
The analytical parameters pH, buffer composition, and amounts of MWCNT-COOH and MIP were investigated and optimized.
Heating rates of uncoated nanoparticles were substantially dependent on buffer composition but less influenced by particle concentration.
Several factors, such as buffer composition, running voltage and capillary temperature were optimised using factorial design and response surface methodology.
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