Sentence examples for buffer complexes were eluted from inspiring English sources
After rinsing five times with the lysis buffer, complexes were eluted in non-reducing sample buffer, resolved by SDS-PAGE, and immunoblotted with mAb 5A6.
After washing in RIPA buffer, complexes were eluted in denaturing sample buffer, resolved by 10% PAGE-SDS and immunoblotted with anti-His antibody (to detect Wnt3a) or with anti-DUF-959 (to detect V3Nter or V2Nter).
After performing six 10 min long washes (2× low salt, 2× high salt, 2× LiCl buffer), complexes were eluted in lysis buffer.
After washing three times with RIPA buffer, immunoprecipitated complexes were eluted in sample buffer by boiling for 5 minutes, electrophoresed through SDS-polyacrylamide gels, and subjected to immunoblot analysis.
The beads were then washed with NP-40 lysis buffer and complexes were eluted by boiling in Laemmli sample buffer.
Following washing twice with PNK buffer, Protein RNA complexes were eluted from the beads using 1× NuPage Loading buffer supplemented with 50 mM DTT at 70°C for 10 min. Protein RNA complexes were then resolved on an 8% Bis-Tris Gel and transferred to nitrocellulose.
The Strep-Tactin Sepharose column was washed five times with 2.5 ml wash buffer, and purified complexes were eluted with 6 ml wash buffer containing 10 mM desthiobiotin (Sigma-Aldrich, St . Louis MO).
The beads were washed using RIPA buffer [58]and protein-DNA complexes were eluted using ChIP elution buffer [59].
The beads were rapidly washed three times with lysis buffer, and the complexes were eluted by boiling in 2× Laemmli sample buffer supplemented with 10% β-mercaptoethanol.
The beads were rinsed several times in IP buffer and immune complexes were eluted in 50 ul SDS PAGE loading buffer and separated as described above for Western blots.
Beads were pelleted by centrifugation at 2700 g for 2 min, washed three times with 300 µl lysis buffer and protein complexes were eluted with 50 µl 2× SDS-sample buffer and analyzed by SDS PAGE and western blotting.
