Sentence examples for buffer complex from inspiring English sources

DAB-chromogen in 1 ml of DAB-substrate buffer complex (DAKO, #K3468) was applied for 5 min for Pecam1 antibody and for 5 15 min for Vegfa antibody.

However, these buffer complexes are not stable and are readily oxidized by Au III).

After rinsing five times with the lysis buffer, complexes were eluted in non-reducing sample buffer, resolved by SDS-PAGE, and immunoblotted with mAb 5A6.

After washing in RIPA buffer, complexes were eluted in denaturing sample buffer, resolved by 10% PAGE-SDS and immunoblotted with anti-His antibody (to detect Wnt3a) or with anti-DUF-959 (to detect V3Nter or V2Nter).

After performing six 10 min long washes (2× low salt, 2× high salt, 2× LiCl buffer), complexes were eluted in lysis buffer.

buffered complex glycerol medium.

Pichia pastoris transformants were grown in 10 ml Buffered Complex Glycerol medium (BMGY; 10 g l−1 yeast extract; 20 g l−1 peptone; 100 mM potassium phosphate buffer pH 6; 1% glycerol; 0.00004% biotin) over night at 30 °C and 180 rpm.

Cells were grown in 50 mL of buffered complex medium in a 250-mL flask in an orbital shaker (250 rpm) at 28 °C to an OD600 of 3 6.

At culture OD600 of 2 6, the cells were harvested by centrifugation and re-suspended in buffered complex methanol medium at an OD600 of 1.0.

Aliquots of cells were then diluted to an OD600 of 1.0 with 200 mL of buffered complex medium containing methanol and incubated for 3 5 days with daily addition of methanol up to 0.5%%.

For recombinant protein production by the methanol utilization plus P. pastoris strain, 25 ml buffered complex glycerol medium (BMGY) followed by 100 to 200 ml BMMY medium was generally used in shaking flask fermentation [ 14].