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For the purification of mutant versions of Sso TFEα/β-His and WT TFEα/β-His serving as control the heparin-affinity chromatography was omitted and instead the protein was buffer changed on a PD-10 column (GE Life Sciences) to TK(150).
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"Things changed on a dime".
It changes on a play.
These buffers can be changed on the fly depending on the requirements of the wireless channel.
The eluted α-amylase was subjected to a buffer change into Tris-HCl buffer (50 mM, pH 7.0).
To obtain an apo-form structure, the Tris buffer was changed to either Hepes or citrate buffer.
This buffer capacity confirms groundwater's ability to buffer changes.
The MCs (buffer, surfactant cosurfactant, oil) and the PVs (voltage, buffer concentration, buffer pH) were simultaneously changed according to a MPV experimental design.
The buffer was changed, 10 μl of receptor/DNA complexes were loaded on to the gel and the gel was run for an additional 60 90 min.
A synthetic medium based on ultra-pure water, trace-elements and phosphate buffer, was changed weekly [ 48]. 300 randomly chosen adult daphnids were collected prior to proteome analysis.
Second, we present a novel on-chip network router with a shared buffer based on a hierarchical bit-line buffer.
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