Sentence examples for buffer change from inspiring English sources

It has been previously noted that different sensor surfaces can respond differently to the same buffer change.

All fractions containing the target protein were pooled and dialyzed against 100 mM HEPES (pH 7.5) overnight at 4 °C with one buffer change.

The collected fractions were subjected to buffer change by ice-cold Homogenization Buffer without sucrose and concentrated by an Amicon Ultra centrifugal filter device (Millipore).

After production and purification as described above, all rat and human full-length or recombinant TrxR and SecTRAP preparations were subjected to desalting with NAP-5 columns (GE) for buffer change to 50 mM Tris-Cl, 2 mM EDTA, pH 7.5 (TE-buffer) and were kept in −20°C at a concentration of approximately 1 mg/ml until use.

A third buffer change was given for overnight dialysis at 4°C.

The purified proteins were stored at −70 °C after buffer change (PBS buffer with 10% glycerol).

This buffer capacity confirms groundwater's ability to buffer changes.

After electrophoresis, the native-PAGE was washed in 20 mM phosphate buffer (pH 8.0) at 4°C and 50 rpm for 90 min, with buffer changes every 30 min.

The precipitate exhibiting trypsin activity was then collected using centrifugation and dialyzed with 0.1 M Tris HCl pH 8.0 (overnight with two buffer changes) at 4°C.

Seagrasses also undergo a high rate of photosynthesis that may serve to buffer changes in ocean chemistry that affect shell-building organisms.

This paper presents a unique quantitative assessment of groundwater storage in different types of hard rocks and a first estimate of the capacity of hard rock aquifers to buffer changes in climatic and anthropogenic conditions.