Sentence examples for buffer cells were washed from inspiring English sources

incubation in perm-wash buffer, cells were washed and acquired on a flow cytometer.

Following erythrocyte lysis using ammonium chloride (NH4Cl) buffer, cells were washed, and resuspended in media (RPMI, 5% FCS, antibiotics).

After incubation in collagenase-free buffer, cells were washed with PBS and then fixed in 100% methanol for 10 min at room temperature.

After 20 min fixation using the BD Cytofix/Cytoperm buffer, cells were washed with and stained in 0.5% w/v Saponin (Sigma) for 20 min on ice.

After stopping digestion with a serum- and calcium-containing buffer, cells were washed with PBS and collected by centrifugation (180 rcf, 1 min).

Following three washes with 8% sucrose in 0.1 M phosphate buffer, the cells were washed again in 0.1 M sodium cacodylate buffer and stained with 1% osmium tetroxide and 0.5% potassium ferrocyanide in 0.5% sucrose for 1.5 h.

After quenching excess biotin with 100 mM quenching buffer, the cells were washed with TBS, and dissolved in 0.8 ml RIPA buffer to create whole cell lysates.

For solutions of protein in buffer, the cells were washed with a solution of 10 mM Tris HCl pH 7.2, 100 mM NaCl, 6.8 μM SbpA and the indicated concentration of MgCl2 or CaCl2.

Single-cell suspensions were prepared from spleen and bone marrow and lysed for red blood cells (RBCs) using Ammonium-Chloride-Potassium (ACK) lysis buffer, and cells were washed with RPMI 1640 supplemented with 5% FCS and later used for flow cytometric analysis.

Subsequently, the reaction buffer was removed, cells were washed and fresh reaction buffer was added.

The incubation was terminated by 10-fold dilution with ice-cold PBS buffer before the cells were washed and submitted to FACS analysis.