Sentence examples for buffer cells were treated from inspiring English sources

Following an overnight incubation in blocking buffer, cells were treated with a 1∶1000 dilution (1∶100 for pericentrin) of primary antibody to blocking buffer for 1 hour.

After washing with SP buffer, cells were treated with Zymolyase 20T in SP buffer for 30 min to digest the cell wall.

After establishing basal equilibrium with the nitrate buffer, cells were treated with CFTR agonists (Fsk and IBMX) prepared in the nitrate buffer and aliquots were collected six times at 1 min intervals.

Cells were fixed in precooled 70%% ethanol (diluted with PBS) for 2 h and then washed with ice-cold PBS twice; after it was suspended with budding buffer, the cells were treated with RNaseA and stained with propidium iodide for 30 min at room temperature.

The supernatant was aspirated and the cell layer was washed twice with ice-cold KRH buffer before the cells were treated for 30 min with 0.5 ml 0.05 N NaOH in phosphate-buffered saline.

After washing with phosphate buffered saline, the confluent cells were treated in 0.25% w/v trypsin and 1 mM EDTA at 37°C for 5 min, followed by adjustment to 1 × 10 cells in 1 mL of DMEM medium.

B. burgdorferi B313 and B313/pBR were processed instantly, whereas B. recurrentis cells were incubated with PLG (10 µg) in the presence or absence of the lysine analogue tranexamic acid (TA); as a negative control, cells were treated with buffer.

Thereafter, cells were treated with buffer or different concentrations of fMLF (10−5 ℳ, 10−7 ℳ and 10−8 ℳ) for 30 min.

To analyse binding of different CXCR2 and CXCR1 antibodies, neutrophils or U937-CXCR2 cells were treated with buffer, 0.5 μM Staphopain A with or without 1 μM Staphostatin A for 15 min at 37°C.

For intracellular fluorescent-activated cell sorter (FACS) staining of rat PLFs, cells were treated with permeabilization buffer (0.1% saponine in phosphate-buffered saline, 0.1 M hepes and 1% FCS) and stained with anti-prolyl-4-hydroxylase (clone 6-9H6; DPC BadrmaNauheim Nauheim, Germany) and anti-CD68 (clone ED1; DPC Biermann), accordingly.

Cells were treated initially with HEPES buffer and then with buffer containing 100 μM C2-ceramide.