Sentence examples for buffer cells were transferred from inspiring English sources
After suspending in breaking buffer, cells were transferred to a glass tube containing glass beads and were vortexed to break the cells.
Non-adherent cells were removed with warm Dulbecco's phosphate buffered saline (DPBS), and the cells were transferred to RPMI containing 10% fetal bovine serum (FBS) for another hour.
Cells (1×107 cells/ml) were resuspended in staining buffer and 100µl cells suspension (1×106 cells) were transferred into each sample tube.
Cells were transferred to lysis buffer either singly or in pools of 20 cells of a single type.
Cells were transferred to lysis buffer directly after sorting and total RNA was isolated using the RNAeasy Microkit (Qiagen, Hilden, Germany) according to the manufacturer's protocol.
Packed red blood cells were transferred to lysis buffer and stored frozen until DNA was extracted.
For the fluorescence-activated cell sorter (FACS) analysis cells were transferred to FACS buffer (PBS, 1% bovine serum albumin, 0.05% NaN3).
Once they reached an OD600nm = 1.0, cells were transferred to PBS buffer and incubated with 1 µM CM-H2DCFDA at 30°C for 60 min. Excess dye was washed three times and cell fluorescence was analyzed by FACS.
After a short spin, lysed cells were transferred to Eppendorf tubes and 250 μl Buffer P2 and 350 μl Buffer N3 was added to each tube.
After collection, the cells were transferred directly into 6.5 μL of CellsDirect buffer (Invitrogen) for Fluidigm or 10 μL RNA direct lyses buffer for microarray analysis.
