Sentence examples for buffer cells were stained from inspiring English sources

Following a wash in FACS buffer, cells were stained with anti-GL7 AlexaFluor 647 (eBioscience, UK) at 1 100 dilution, anti-CD95 PE (eBioscience, UK) at 1 100 dilution and anti-B220 PeBiosciencecience, UK) at 1 200 for 30 min at RT in the dark.

After incubation with the blocking buffer, cells were stained with anti-BrdU antibody (1 100; BD Biosciences, Franklin Lakes, NJ, USA) overnight at 4°C.

After washing twice with staining buffer, cells were stained with directly labelled anti-CD3 for 30 min on ice, washed and then fixed with 1% paraformaldehyde.

After washing with Annexin V binding buffer, cells were stained with 2000-fold diluted Cy5-labeled AnnexinV (Biovision, Milpitas, CA) and 500 nM Sytox blue (Invitrogen) and analyzed by flow cytometry.

After washing twice with 500 μl PBA buffer, cells were stained with Alexa-Fluor-488 coupled mouse anti-penta-His antibody (Qiagen) or rabbit anti-exotoxin A polyclonal antibodies and fluorescein isothiocyanate (FITC -labeled FITC -labeledents oF ab' 2lonal goat anti-rabbit antibodies (Sigma-Aldrich).

After incubation in fix/perm buffers, cells were stained for intracellular antigens.

After washing with FACScan buffer, the cells were stained with phycoerythrin-conjugated anti-mouse CD8a antibody.

After washing cells with one times permeabilization buffer twice, cells were stained with anti-human FOXP3-FITC antibody for 30 minutes at 4°C in the dark.

After further washing by rinse buffer, the cells were stained with the Alexa Fluor® 488 dye-labeled anti-BrdU antibodies for half an hour.

After the cells were washed three times with PBS buffer, the cells were stained with Prussian blue solution containing 20% (v/v) hydrochloric acid and 10% (v/v) potassium ferrocyanide solution or MRI phantom observation.

Following incubation with IC fixation buffer (eBioscience), cells were stained intracellularly with anti-IFN-γ PECy7, anti-IL-10 allophycocyanin, anti-IL-17A PE, and/ or anti-IL-4 PE (all eBioscience).