Sentence examples for buffer cells were resuspended from inspiring English sources

After washing twice with the wash buffer, cells were resuspended in 100 µl wash buffer, transferred in a microtiter plate and fluorescence intensity was measured at excitation 485 nm and emission 530 nm.

After washing with cold buffer, cells were resuspended in 300 μl of PBS for FACS analysis by FACScan (Becton Dickinson).

After washing with 0.5 ml of 1x BD Perm/Wash buffer, cells were resuspended in 0.5 ml of 1x PBS containing 0.02% sodium azide, 250 μg/ml RNase A and 2 μg/ml of 4′,6-diamidino-2-phenylindole (DAPI), then incubated at 37°C for 30 min in the dark.

After the mixture was washed twice with 1.2 mL isolation buffer, the cells were resuspended in 300 μL isolation buffer; 500 μL pre-washed Dynabeads were added followed by incubation for 30 min on a gentle rotating shaker at room temperature.

Following two washes with washing buffer the cells were resuspended in 1 mL of washing buffer.

Erythrocytes were lysed using NH4Cl buffer, and cells were resuspended in media.

After washing with the same buffer the cells were resuspended with PBS-0,1% FCS, and analyzed.

After washing in 1× PermWash buffer twice, cells were resuspended in small droplets of glycerol, the cell suspension carefully mixed, transferred onto microscope slides, and covered with coverslips.

After removing the fixation buffer, the cells were resuspended in PBS and allowed to adhere to 0.1% poly-l-lysine-coated microscope slides for 10 min at room temperature.

After one wash with buffer and with Annexin V-buffer (PharMingen), the cells were resuspended in 100  μl of Annexin V-buffer and incubated with 1  μl of the AnnexinV-FITC conjugate (PharMingen) for 20 min at RT in the dark.

After centrifuged and lysized in RBC lysis buffer, the tumour cells were resuspended in RPMI1640 medium with 10% FBS, penicillin and streptomycin.