Sentence examples for buffer cells were reacted from inspiring English sources

After blocking with the blocking buffer, cells were reacted with the anti- C. paradoxa DipM antibodies (1 100 in the blocking buffer) for 4 h at room temperature.

After washing with PBS-T four times, cells were reacted with 1.4-nm gold particle-conjugated secondary antibody (1 80 in blocking buffer) at 4˚C overnight.

After being washed twice, the cells were reacted with UEA-1 (10 mg/l) for 1 hour.

Cells were reacted with markers for astrocytes (GFAP), neurons (TuJ1), and oligodendrocytes (O4).

Cells were reacted with primary antibodies (described below) overnight at 4°C.

Pretreated cells were reacted with DCFH-DA according to the manufacture's protocol.

As controls for nonspecific staining, cells were reacted with appropriate isotypically matched irrelevant murine antibodies.

Following FGE treatment, Fcs were buffered exchanged into labeling buffer and were reacted with AF488 hydroxylamine as stated above.

After the membranes were washed with TBST buffer, they were reacted with the appropriate horseradish peroxidase-conjugated secondary antibodies for 30 min at room temperature.

Cell suspensions were reacted with M2VP (1 mM) to determine GSSG.

Cell lysates were reacted with az-rho or az-biotin via click chemistry.