Sentence examples for buffer cells were processed from inspiring English sources

Following the addition of 400 μl binding buffer, cells were processed within 1 h using the FACScan flow cytometer Coulter XL (Beckman Coulter).

After 48 hr, NRP-152 and NRP-154 cells replicate wells of cells were harvested with either trypsin/EDTA (GIBCO) or 0.15 M NaCl/ 0.01 M Na citrate buffer (citrate-saline buffer), and the cells were processed for intracellular flow cytometry to analyze IL-6 production, as described above.

Unless otherwise indicated, mice were killed 12 weeks after transplant, and the cells from both the tibiae and femurs of each mouse were collected into cold phosphate-buffered saline containing FCS. Red cells were lysed with ACK lysing buffer (Invitrogen) and residual white blood cells were processed for flow cytometry as described above.

Cells were processed in PEM-based buffers (200 mM Pipes, 2 mM EGTA, 2 mM MgSO4, adjusted to pH 6.9).

Following treatment, cells were processed as described previously [ 39] in Cell Extraction Buffer (BioSource International, Inc., CA).

Cells were processed immediately after isolation.

incubation in perm-wash buffer, cells were washed and acquired on a flow cytometer.

Afterwards cell lysates were processed as above with centrifugation in the sucrose buffer.

Cell lysates were processed for Western Blotting.

Cell pellets were processed for SDS-PAGE.

For GSH determination, cells were homogenized in PEN buffer without reducing agents and were processed immediately as described below.