Sentence examples for buffer cells were perfused from inspiring English sources

After a basal record of fluorescence in Cl−-containing HEPES buffer, cells were perfused with a chloride-free buffer to induce a chloride efflux from the cytoplasm and subsequently exposed to HEPES buffer to allow the chloride readmission.

Subsequently, cells were gently perfused with buffer containing Ca2+ and after 6 8 min cells were perfused with physiological buffer including CDCA or GPBAR-A.

Before drug application, to acquire a stable baseline, RA-differentiated SH-SY5Y cells were perfused with buffer solution for 5 min and for 25 min with buffer solution to control, and for 25 min for other drugs dissolved in buffer solution.

Cells were perfused with buffer (normal or minimal Ca2+) for 10 min.

To assess the effects of nephrin on the FFA-induced release of Ca2+ from intracellular stores, GEnC cells were perfused with buffer containing minimal Ca2+ and stimulated with FFA.

Control experiments were first established whereby cells were perfused with KHM buffer until a steady fluorescence baseline had been achieved (Figure 2B(i), CaBP7 and Figure 2B v), CaBP8).

To evaluate the potassium depolarization amplitude, cells were perfused with Krebs buffer and stimulated with KCl 50 mM for 2 minutes.

To assess the ghrelin and leptin induced response, cells were perfused with Krebs buffer for 12 minutes and afterwards stimulated with ghrelin (10−10 M; Peptide Institute, INC) or leptin (10−12 M; Phoenix Pharmaceuticals) for 20 minutes.

Cells were perfused with saline buffer (plus glucose as indicated) at room temperature.

Capan-1 cells were perfused with a buffer containing 5 mM Na+, which favours Ca2+ influx via NCX as observed in Fig.  9c.

These oscillations decreased progressively in amplitude when cells were perfused in LC buffer, consistent with emptying of intracellular stores which were then unable to refill through the store-operated Ca2+ entry route [ 49].