Sentence examples for buffer cells were incubated from inspiring English sources

After incubation for 60 min in blocking buffer, cells were incubated with primary antibodies for 60 min at room temperature or overnight at 4°C (primary antibody IgG was diluted into PBS with 0.05% Triton X-100 and 0.2% BSA).

Following two washes in cold wash buffer, cells were incubated with mouse monoclonal anti-thioredoxin (ab16965; AbCam) or IgG2b isotype control antibody (ab91366; AbCam) on ice for 30 minutes.

After three consecutive washes in wash buffer, cells were incubated for 45 min with secondary antibodies (Alexa Fluor Donkey-anti-mouse and Alexa Fluor Donkey-antInvitrogenInvitogethertogether with Hoechst 33342 (Invitrogen).

Following washing with binding buffer cells were incubated with a rabbit anti-human C4BP antibody, followed after washing with swine anti-rabbit FITC labeled antibodies (DAKO).

After 30 min in 1N HCl and neutralization with sodium borate buffer, cells were incubated with monoclonal anti-BrdU antibody (1∶100, Chemicon International) in PBS + 0.1%Triton + 5% goat serum for 3 hours.

After removing Cytofix/Cytoperm Buffer, cells were incubated with 100 µL of diluted DNase (diluted to 300 µg/mL in PBS) for 1 hour at 37°C to expose incorporated BrdU.

After washing, centrifuging, and suspending in 500  μL of 25 mM phosphate buffer pH 7.0 (buffer A), cells were incubated for 30 min in the dark with 0.02 mM 2,7 dihydrodichlorofluorescein diacetate (H2DCFDA).

After rinsing with buffer A, cells were incubated with lysis buffer (1% nonidet P-40, 4 mg/ml dodecyl-β-D-maltoside, 0.8 mg/ml cholesteryl hemisuccinate in buffer A) plus protease inhibitors for one hour on ice.

For buffer extracts, cells were incubated with 0 (buffer only), 0.5, 1, 1.5 and 2 μg of total protein extracts from each plant.

The caps containing buffer and cells were incubated upside-down at room temperature (RT) for 30 min and then briefly centrifuged to collect the buffer and cell complexes.

After washing twice with Phosflow Perm/Wash Buffer I, cells were incubated with phycoerythrin-conjugated goat anti-mouse IgG antibody (BD Bioscience) to detect 4B6 or phycoerythrin-conjugated avidin (BD Bioscience) to detect biotin-conjugated anti-PIP3 antibody.