Sentence examples for buffer cells were fixed from inspiring English sources

After washing twice in FACS buffer cells were fixed in 300 µl 1% p-formaldehyde in PBS.

After washing two times with the FACS washing buffer, cells were fixed with 1% ice cold paraformaldehyde and analyzed by flow cytometry.

After washing in KHM buffer, cells were fixed and permeabilzed with ice cold methanol for 5 min at −20°C and blocked with 3% BSA for 1 h at room temperature.

After washing with FACS (Fluorescence Activated Cell Sorter) buffer, cells were fixed with 4% paraformaldehyde and analyzed on a FACS Calibur using Cell Quest and FlowJo 7.6.1.

After washing with FACS buffer, cells were fixed with 1% (weight/volume) paraformaldehyde for 30 min and then stored in the dark until analyzed by flow cytometry.

After washing with PBS buffer, cells were fixed, permeabilized, and stained with Alexa Fluor 568 phalloidin (Molecular Probes, Eugene, OR, USA), FITC conjugated anti-human CD41a antibody (Dako cytomation) and DAPI.

Following incubation of the transfected and non-transfected cells with 50 μl FcR blocking reagent (Miltenyl Biotec) for 15 min at 4°C and washing in 10 ml staining buffer, the cells were fixed in 500 μl IC Fixation buffer (eBioscience, Frankfurt, Germany) in the dark for 20 min.

After washing with 0.1 M cacodylate buffer, the cells were fixed with 1% OsO4 in the same buffer, stained with 1% uranyl acetate in 0.1 M maleate buffer (pH 5.2), dehydrated in ethanol, and embedded in Poly/Bed 812 (Polysciences, Warrington, PA).

Following incubation, the cells were washed three times with FACS buffer and cells were fixed in 200 µl of 2% ultrapure formaldehyde (Polysciences, Inc., Warrington, PA) diluted in FACS buffer (fixation buffer).

After 3 washes with FACS Buffer the cells were fixed in cold 2% Paraformaldehyde in PBS and 10,000 Thp-1 cells were acquired using a FACSCalibur flow cytometer.

Following incubation, the cells were washed three times with FACS buffer and cells were fixed in 200 µl of 2% ultrapure formaldehyde (Polysciences, Inc., Warrington, PA).