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After a 10 minutes recovery period in Krebs buffer, cells were depolarized with KCl 50 mM for 2 minutes.
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Cells were depolarized by extracellular potassium and the depolarization-induced calcium (Ca2+) influx was quantified by using Fura-2 imaging.
Cells were depolarized by voltage steps from −80 to +60 mV in 10-mV increments (Δ10 mV).
Cells were depolarized by voltage steps from −80 to +60 mV in 10-mV increments (Δ10 mV, upper panel).
When osteogenic cells were depolarized after pre-differentiation, four out of five donors exhibited lowered IBSP expression and four out of five donors exhibited lower calcium content compared to non-depolarized cells in four out of five donors.
After [Ca2+]i returned to the basal level, the cells were depolarized for 3 s in the presence of 4 mmol/L Ca2+ to boost the Ca2+ influx.
Conversely, when the cells were depolarized there was a clear reduction of the rebound response (Fig. 6D).
Further, the cells were depolarized (−41.3 ± 0.56 mV) and fired tonically at 38.1 ± 7.4 Hz.
Cells were depolarized with field potentials of approximately 10 V/cm for 1 ms via platinum iridium electrodes.
We confirmed that when NVmes cells are depolarized by current injection, firing occurs during the depolarizing crests of the oscillations [19].
In this process, H+ accumulates inside microglia and the cells are depolarized by the electron current.
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