Sentence examples for buffer cells were cultured from inspiring English sources

After red blood cell lysis using Tris-NH4Cl buffer, cells were cultured in RPMI (containing 1% penicillin/streptomycin and 10% fetal calf serum [FCS)] and stimulated with total OVA (10 μg/mL) or OVA peptides (10 μg/mL) that are specific for MHC class I (OVA 257) or MHC class II (OVA 323) for 48 h.

Fig. 3 Pyruvate and PHB production by Halomonas sp. KM-1 in the presence of different concentrations of sodium bicarbonate buffer KM-1 cells were cultured under aerobic conditions (agitation speed: 200 rpm) at 40 °C.

Briefly, RAW 264.7 macrophages or CT26 tumor cells were cultured in migration buffer (media with BSA rather than serum) for 24 hrs to generate conditioned buffer (CB).

Cells were cultured in buffered RPMI 1640 supplemented with 10% (LCL721) or 15% (LCL1261) fetal bovine serum and 50 mg/mL gentamycin.

Coverslips were assembled into temperature-controlled chambers and cells were cultured in Ringers buffer [24].

After 22 hours, the media were removed and cells were cultured in EBSS buffer (120 mM NaCl, 5.4 mM KCl, 0.81 mM MgSO4, 1 mM NaH2PO4, 5.5 mM D-glucose, 0.2 mM CaCl2, 25 mM HEPES) containing the indicated additives.

Methods: Bovine aortic endothelial (BAE) cells were cultured, detached, then suspended in buffer and their mechanical and geometrical properties studied with the EchoCell system.

Cells were cultured on coverslips and washed with phosphate buffered saline (PBS) prior to fixation.

(B ) 293T cells were cultured for 24 hr and then harvested and lysed in Buffer II.

Cells were cultured for 24 h and then lysed in passive lysis buffer.

LNCaP cells were cultured, treated, and harvested as described above and resuspended in Calcium Buffer.