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After washing twice in 1x permeabilization buffer cells were analysed by flow cytometry as described above.
After washing with 1 ml of 1X permeabilization buffer, cells were analysed by flow cytometry as described above.
After adding annexin, buffer cells were analysed by a FACS Canto II flow cytometer (BD Biosciences).
After washing in Perm/Wash Buffer cells were analysed by FACScalibur flow cytometer using CellQuest software.
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After additional washing in annexin-binding buffer, the cells were analysed for annexin V-FITC binding in a FACSCalibur TM flow cytometer using CellQuest software (Becton and Dickinson, San Jose, CA, USA).
After incubation, 400 μl of binding buffer were added and cells were analysed by flow cytometry with FACScalibur (Becton Dickinson) and data were processed using FlowJo software, determining the percentage of apoptotic cells.
Four hundred microlitres of 1× binding buffer was added, and the cells were analysed by flow cytometry (BD FACS Calibur) within 1 h.
At the end of the incubation, 400 μl of binding buffer was added to the tube, and the cells were analysed immediately by flow cytometry.
1 × 104 cells were analysed per sample.
In each image, 2000 3000 cells were analysed.
Cells were analysed by flow cytometry.
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