Sentence examples for buffer can be lyophilized from inspiring English sources

Pluronic lysis buffer can be lyophilized to dryness, stored as a dry reagent, and readily dissolved in H2O as required.

Pluronic-based buffers can be lyophilized, stored in dry form, and easily hydrated.

Finally, the protein was lyophilized and dissolved in sample buffer for SDS-PAGE.

Annealed DNA oligos (Eurogentec, Liege, Belgium) were lyophilized and dissolved in NMR buffer to a stock concentration of 11.5 mM.

The crude lipase from supernatant of the culture was lyophilized and redissolved in 50mM Tris HCl buffer (pH 8.5).

During enzyme assays, the T1, T2, and T3 prehydrolyzates were lyophilized and mixed with 100 mM buffer solutions to raise the pH to 4.8.

The resulting filtrate was lyophilized and resuspended in 50 mM phosphate buffer at pH 7.4 in D2O for NMR measurements.

For thioflavin-T fluorescence assays, aliquots were lyophilized and redissolved in 20 mM Tris buffer, pH 7.4, at the desired concentration.

To extract RNA, the frozen cell pellets were lyophilized using liquid N2 and resuspended in buffer RLT (RNeasy Mini Kit, Qiagen Inc., Valencia, CA).

The gel pieces were extracted twice and the pooled digest was lyophilized and re-suspended in the aqueous buffer (10 μL of 1% formic acid/2% acetonitrile).

The sample was lyophilized and prepared in 500 μL of buffer containing 10 mM NaH2PO4, 100 mM NaCl, and 5 μM Na2EDTA (pH 7.0).