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A detailed description of western blotting of whole-cell lysates extracted in RIPA buffer can be found in Supplementary Methods.
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Detailed descriptions of the experiments (including buffer solution compositions) can be found in the Supplementary material online.
If the heterointerface formed on this surface, then the IF bonds configuration is different from the film because there are mixed In As and Ga As bonds in the IF layer;however, only Ga As bonds can be found in the buffer and only In As bonds can be found in the film.
(iii) When a packet leads to link failure detection due to three consecutive, unsuccessful layer three transmission attempts, it is buffered and transmitted if a new route can be found.
When a packet leads to link failure detection due to three consecutive, unsuccessful layer three transmission attempts, it is buffered and transmitted if a new route can be found.
If no fitting target segment can be found in the current buffer, it is stepwise expanded until a respective number of target segments can be found [40].
Since the latency can be derived directly from the maximum buffer size, the reason why at these points a minimum latency can be found is the same.
More details on the buffer concentrations used and the specific procedure for each specific kinase assay can be found in the KinaseProfiler Service Assay Protocols [45].
The setup for the photonic buffer is schematically shown in Fig. 1c) (a detailed description of the setup can be found in the 'Methods' section and in Supplementary Fig. 1).
Primer sequences can be found in Supplementary Table 2. Cells were lysed in 2 × Laemmli buffer.
Full gels can be found in Supplementary Fig. 9. Whole-cell lysates and nuclear lysates were prepared using RIPA buffer and RSB buffer (10 mM Tris-HCl (pH 7.5), 10 mM NaCl, 3 mM MgCl2, and inhibitors), respectively, as previously described19.
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