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DNA was also extracted manually with Triton X lysis buffer by the method used in the previous study for DNA extraction from bacterial colonies [ 13].
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Of note, the method was further applied for the detection of thrombin in human serum samples, showing the results in agreement with those values obtained in an immobilization buffer by the colorimetric method.
The genomic DNA of safflower genotype SU was isolated from fully expanded leaves using CTAB buffer following the method described by Paterson et al. (1993).
The size of the project buffer determined by the proposed method is more reasonable, thus signifying that it can doubly optimize project duration and cost.
In another set of experiment, immediately after euthanasia the stomachs were quickly removed, homogenized (1 10 wt/vol) in 0.5% hexadecyltrimethyl ammonium bromide Sigma-Aldrichh Chemical Company, U.S.A) in 50 mmol potassium phosphate buffer (pH 6.0) and assayed by the method of Bradley et al. [ 12] as described by Tariq et al. [ 13].
The pellet obtained was re-suspended in Tris-HCl buffer, and protein content was determined by the method of Lowry.
The intracellular pH buffering capacity due to non-bicarbonate buffers present in muscle was determined by the method of Castellini & Somero (1981) as modified by Blomberg & Baldwin, (1991).
Rewriting-logic environments such as ELAN, the one we use here, are sufficiently versatile to allow for adequate specifications and simulations which through easy modifications of the strategies enable a dynamic verification of aspects intrinsically related to hardware properties such as the size and control of reorder buffers and the method of predictions used by speculative processors.
The erythrocyte membranes were prepared by the method of Dodge et al. [ 30] with Tris HCl buffer.
Protein concentrations were determined by the method of Bradford [ 95] and sample volumes were equalised with label-free solubilisation buffer.
The purified protein with urea was then refolded in 20 mM Tris buffer by drop dilution method (Howarth et al. 2006).
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