Sentence examples for buffer by running for from inspiring English sources

Amplicons of the multiplex PCR were analyzed by electrophoresis on MetaPhor™ agarose gels (3%, Cambrex Corporation) in TAE buffer by running for 16 h at 25 V and subsequent ethidium bromide staining.

PCR products were analyzed by electrophoresis using 2% agarose gel (Promega, USA) containing ethidium bromide in 1× TBE buffer, and run for 1 hr with 70V, and visualized by UV Trans-illuminator (UVP) and Gel Documentation System (UVP).

Agarose gels (0.8%, 12 cm) cast in 10 mM sodium phosphate buffer were run for 2 hours at 100 volts with the buffer recirculated by a peristaltic pump.

To correct for refractive index changes caused by running buffer and instrument noise, running buffer was injected at a flow rate of 30  μl/min in the blank flow cells and the observed response difference was subtracted from the experimental data.

The capillary was rinsed with 1 M NaOH followed by running buffer to prevent carryover between runs.

The capillary was rinsed with 1 M sodium hydroxide followed by running buffer to prevent carryovers.

In our study, the capillary was rinsed after each run with 0.1 mol/L sodium hydroxide for 2.0 min, with water for 2.0 min followed by running electrolyte (phosphate buffer at pH 7.4) for 3.0 min (pressure = 3000 mbar each).

Peptides were eluted by running a 0 20% buffer B gradient for greater than 55 min. and 20% 100% buffer B (20% v/v ACN, 5 mM KH2PO4 pH 3.2, 500 mM KCL) for 20 min. at a column flow rate of 50 µl/min.

The baseline was subtracted by running HEPES buffer (pH 7.4) as a blank.

The apparent optimum pH of the enzyme was determined by running the standard assay using 50 mM Tris HCl buffer or 50 mM DEA buffer for the pH ranges 7.0 8.5 and 7.5 10.5, respectively.

RhoA levels were quantified by running bead/protein complexes in loading buffer containing 0.1 M DTT.