Sentence examples for buffer by boiling for from inspiring English sources

Immunoprecipitates were extensively washed with IP wash buffer (10 mM Tris at pH 7.5, 150 mM NaCl, 1 mM EDTA, 0.2% Triton X-100) supplemented with 1× phosphatase inhibitor mix (Pierce) and 1× protease inhibitor mix (Roche), and then eluted with SDS PAGE loading buffer by boiling for 5 min.

The protein complexes were then eluted from the beads in 30 µL of 2× SDS-PAGE sample buffer by boiling for 10 min. Samples were separated on SDS-PAGE and transferred to PVDF membranes for Western blotting.

Immunoprecipitated complexes were washed four times with RIPA buffer and eluted in sample buffer by boiling for 5 minutes.

After washing three times with RIPA buffer, immunoprecipitated complexes were eluted in sample buffer by boiling for 5 minutes, electrophoresed through SDS-polyacrylamide gels, and subjected to immunoblot analysis.

Protein samples, 30 µg of soluble extract or cell number equivalent of insoluble pellet, were reduced in 6× sample buffer by boiling for 5 minutes and resolved on a 7.5% SDS-PAGE gel.

The immuno-complexes were washed four times with RIPA buffer, and proteins were eluted with 2X SDS sample buffer by boiling for 5 min. Ten µl precipitated proteins were resolved on SDS PAGE and subjected to Western blot analysis using the respective antibodies.

After the purified complexes were washed three times with lysis buffer, they were solubilized in Laemmli buffer, followed by boiling for 5 min at 100°C.

The proteins were eluted in 2 × Laemmli buffer (Bio-Rad) or 4 × LDS sample buffer (Invitrogen) by boiling for 5 min.

The beads were then thoroughly washed with lysis buffer containing 20 mM imidazole and resuspended in 5X SDS sample buffer followed by boiling for 15 min. The samples were resolved on SDS-PAGE followed by autoradiography.

The reaction was terminated by the addition of 40 µl 2×SDS sample buffer followed by boiling for 5 min. Samples were resolved by SDS-PAGE gel.

At each time point, aliquots of the reactions were stopped by the addition of 2X SDS-PAGE loading buffer followed by boiling for 4 min. The samples were analyzed by SDS-PAGE, toansferred to a PVDF membrane, and submitted for N-terminal sequencing (Midwest Analytical Inc., St . Louis.