Sentence examples for buffer block is used from inspiring English sources
The row buffer block is used to store the pixel information in the previous row.
Antigen retrieval procedure consisting of an incubation of 30 min 95°C in sodium citrate buffer before blocking was used to enhance M-Cadherin labeling.
Clinical specimens that had been fixed in 10% buffered-formalin and prepared as FFPE tissue blocks were used to generate four-micron sections.
Blocking buffer was used to avoid non specific binding.
The washes were performed between each step and the blocking buffer was used for dilution of each of the reagents that were overlaid onto the tissue sections.
Alexa fluor 647 conjugated streptavidin (1∶100 in blocking buffer) was used to detect 5-BP crosslinked to tissue sections, and Alexa fluor 488 anti-rabbit IgG and Alexa fluor 568 anti-mouse IgG secondary antibodies (1 1000 in blocking buffer) were used to detect primary antibody staining.
For both primary antibodies, goat anti-rabbit immunoglobulin G conjugated with horseradish peroxidase (Pierce; Product Code: 31463); 1∶10000 in blocking buffer) was used as the second step antibody, and ChemiGlow reagent (Alpha Innotech) was used as a substrate.
Alexa Fluor 488-labeled goat anti-rabbit IgG (Invitrogen/Molecular Probes, Eugene, OR) diluted 1∶2000 and fluorescent nucleic acid stain, 4'6-diamidino-2-phenyl-indole dihydrochloride (DAPI) (Invitrogen/Molecular Probes) diluted to a final concentration of 0.25 µg/ml in blocking buffer were used to detect antibody binding and the presence of spirochetes, respectively.
Blocking buffer was used to dilute primary and secondary antibodies.
Rabbit-anti-CBM (diluted 1 3,000 in blocking buffer) was used as the primary antibody for detection of the interaction.
