Sentence examples for buffer before the run from inspiring English sources

Endogenous peroxidase was blocked for 20 min. in 0.003% H202/100% methanol and then transferred to reaction buffer before the run commenced.

Tissue was washed with Tween 20 detergent/TBS buffer before the run was started; 3% hydrogen peroxide was used to block endogenous peroxidase and a TBS-buffered solution of 1 10 normal serum was used for protein block.

The results showed that, compared with the run without cornstalk buffer strip, the run with continuous cornstalk buffer strip in three successive rainfall events reduced sediment yield by 29.1% while the other two runs with cornstalk buffer strip in a single event only reduced sediment yield by 2.0% 9.1%, and early buffer run had a larger reduction in soil erosion than late buffer run.

This was followed by a 30-min incubation in neutral comet running buffer before the slides were put in an electrophoresis tray to allow DNA to migrate for 45 min.

The experiments were performed at 25°C with the HBS-EP buffer as the running buffer.

That half-children, half-adult buffer before the news".

Paillaugue converted and then kicked a penalty as Montpellier opened up a seven-point buffer before the Sharks responded.

EGFP and Emerald tagged proteins were immunoprecipitated using a mouse GFP antibody (Roche #11814460001) and Protein-G magnetic beads (Life Technologies) for 2 hr at 4C. Immunoprecipitated proteins were washed 4 times with ice cold RIPA buffer before running on 4 12% NuPAGE Bis-Tris gradient gels (Life Technologies).

Cells were harvested at various time points (BV2 cells at 1, 2, 4, and 7 h; cortical neurons at 2, 4, and 11 h) with 0.25% trypsin for 3 min and fixed with 4% paraformaldehyde for 10 min, then resuspended in Hank's balanced salt solution with 1% FBS and 1 mmol/L EDTA buffer before running on a flow cytometer MACSQuant VYB (Miltenyi Biotec, San Diego, CA).

For all SPR measurements, the recombinant domain was dialysed in the buffer with the desired NaCl concentration and pH, and centrifuged immediately before the runs to minimize possible effects from non-specific aggregation.

The surplus, he said, provides an important buffer should the economy run into trouble.