Sentence examples for buffer before the reaction from inspiring English sources

For the caspase-3 assay, 1 × 10 Vero cells were resuspended in cold cell lysis buffer before the reaction mix was added.

Probe was diluted with the TE buffer (1 : 1) before the reaction.

Briefly, sections were post-fixed and equilibrated in terminal transferase buffer before the addition of reaction buffer containing digoxigenin-dNTP oligonucleotide.

Endogenous peroxidase was blocked for 20 min. in 0.003% H202/100% methanol and then transferred to reaction buffer before the run commenced.

Inactivation reactions containing 10 mM CaCl2 and 100 mM of buffer (pH 6.5 9.0) were incubated with 5.0 μM PAD2 at 37 °C for 10 min before adding either iodoacetamide or 2-chloroacetamidine (dissolved in 50 mM buffer) to initiate the reaction (60 μL final volume).

The sections were then washed again in distilled water, treated with terminal deoxynucleotidyl transferase, before the reaction was stopped by incubation in stop/wash buffer.

Although the enzyme requires oxygen, in air-saturated buffer we have found that only 1 2 equivalents of product are formed, relatively rapidly, before the reaction nearly ceases.

That half-children, half-adult buffer before the news".

To evaluate the role of organic solvents on heme crystallization, different concentrations of DMSO or PEGs were added previously to sodium acetate buffer before reactions were started.

Cells were washed twice with Tdt reaction buffer before capping the 3′OH ends with biotinylated- ddUTP, blocking with avidin and biotin.

As a negative control, 50 mM 1,10-phenanthroline, a MMP inhibitor, was added to the reaction buffer before applying the FITC-conjugated DQ gelatin to frozen sections.